Vitamin D administration, cognitive function, BBB permeability and neuro-inflammatory factors in high-fat diet induced obese rats.
Int J Obes (Lond). 2017 Jan 17. Epub 2017 Jan 17. PMID: 28093570
OBJECTIVES: The purpose of this research is to investigate the effects of vitamin D administration on cognitive function, nuclear factor Kappa-B (NF-kB), brain derived neurotrophic factor (BDNF) concentration in the hippocampus and blood-brain barrier (BBB) permeability in high-fat diet (HFD) induced obese rats.
METHODS: Male Wistar rats were fed either a control diet or HFD for 16 weeks (n=20); then each group was randomized into two subgroups supplemented orally with 500 IU/kg vitamin D for 5 weeks. A Morris Water Maze (MWM) test was performed at the 21st week to examine cognitive function. BBB permeability was characterized by Evans blue dye in the hippocampus. BDNF and NF-κB concentrations in the hippocampus and serum vitamin D concentrations were also measured.
RESULTS: HFD led to a significant delay in escape latency time and reduced time of MWM probe test due to increased NF-kB and decreased BDNF concentrations in the hippocampus. Vitamin D supplementation in the HFD group significantly reduced body weight, NF-κB concentrations, BBB permeability, and increased BDNF concentrations in the hippocampus.
CONCLUSIONS: Vitamin D reversed HFD-induced cognitive impairments by reduction of the NF-kB and elevation of BDNF concentrations and modulation of the BBB permeability in rats' hippocampus.International Journal of Obesity accepted article preview online, 17 January 2017. doi:10.1038/ijo.2017.10.