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Abstract Title:

Apoptosis Induction in Primary Human Colorectal Cancer Cell Lines and Retarded Tumor Growth in SCID Mice by Sulforaphane.

Abstract Source:

Evid Based Complement Alternat Med. 2012 ;2012:415231. Epub 2011 Jul 17. PMID: 21804859

Abstract Author(s):

Ming-Jenn Chen, Wei-Yu Tang, Che-Wei Hsu, Ya-Ting Tsai, June-Fu Wu, Chen-Wei Lin, Ya-Min Cheng, Yi-Chiang Hsu

Article Affiliation:

Division of Traumatology, Department of Surgery, Chi Mei Medical Center, Tainan, Taiwan.

Abstract:

We have investigated the anticancer effects of the dietary isothiocyanate sulforaphane (SFN) on colorectal cancer (CRC), using primary cancer cells lines isolated from five Taiwanese colorectal cancer patients as the model for colorectal cancer. SFN-treated cells accumulated in metaphase (SFN 6.25 μM) and subG1 (SFN 12.5 and 25 μM) as determined by flow cytometry. In addition, treated cells showed nuclear apoptotic morphology that coincided with an activation of caspase-3, and loss of mitochondrial membrane potential (ΔΨm). Incubations at higher SFN doses (12.5 and 25 μM) resultedin cleavage of procaspase-3 and elevated caspase-2, -3, -8, and -9 activity, suggesting that the induction of apoptosis and the sulforaphane-induced mitosis delay at the lower dose are independently regulated. Daily SFN s.c. injections (400 micromol/kg/d for 3 weeks) in severe combined immunodeficient mice with primary human CRC (CP1 to CP5) s.c. tumors resulted in a decrease of mean tumor weight by 70% compared with vehicle-treated controls. Our findings suggest that, in addition to the known effects on cancer prevention, sulforaphane may have antitumor activity in established colorectal cancer.

Study Type : Animal Study

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