[Studies on mechanism of myricetin-induced apoptosis in human hepatocellular carcinoma HepG-2 cells].
Zhongguo Zhong Yao Za Zhi. 2010 Apr ;35(8):1046-50. PMID: 20617691
OBJECTIVE: To study the mechanism of myricetin inducing the HepG-2 cell line apoptosis.
METHOD: The MTT method was employed to study myricetin pharmacodynamics in HepG-2. The light microscope and transmission was used to identify the tumor cell apoptosis in the morphology. The FCM method and the kit of caspase 3, caspase 9 were hired to detect the apoptosis rates, the content of mitochondrial membrane electric potential and the activity of caspase in cancer cells.
RESULT: Myricetin significantly inhibits the proliferation and induces the apoptosis of HepG-2 in a dose-dependent manner, which is accompanied with G2/M and S phase arrest. In addition, myricetin also increases the activation of caspase 3,9 and results in a depolarization and delta psi m collapse in a dose-dependent manner.
CONCLUSION: The molecular pathway of apoptosis of human hepatocellular carcinoma cell lines induced by myricetin might deal with the mitochondria-mediated pathway.