Structure-activity Relationship Analysis on Antioxidant and Anticancer Actions of Theaflavins on Human Colon Cancer Cells.
J Agric Food Chem. 2018 Nov 26. Epub 2018 Nov 26. PMID: 30474978
The roles of natural products as effective cancer prevention and therapeutic agents have been documented by various studies in recent years, but the action mechanisms and structure-activity relationship need more elucidation. The present study showed that theaflavins (theaflavin and its derivatives, TFs) from black tea caused an inhibitory effect on the proliferation of human colon adenocarcinoma cancer SW480 cells and human colon cancer SW620 cells (IC50<32.0μM) by the induction of cell cycle arrest, but exerted lower toxicity against normal cells with high safety index (1.89 - 6.26). Moreover, TFs triggered a decrease in reactive oxygen species (ROS) in SW480 cells due to their excellent radical-scavenging ability (e.g., IC50 value of TF4 to ABTS•+was 1.91 ± 0.21 μM). More importantly, the structure-activity relationship analysis of TFs exhibited that the galloyl group was important factor to affect these activities. Taken together, we revealed that the TFs could act as substitutes for natural antioxidants and promising anticancer agents with beneficial influence on human health, and then anticipated that this study may provide useful information on the development of therapeutic natural products.