Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

Abstract Title:

Resveratrol Defends Blood-brain Barrier Integrity in Experimental Autoimmune Encephalomyelitis Mice.

Abstract Source:

J Neurophysiol. 2016 Aug 17:jn.00510.2016. Epub 2016 Aug 17. PMID: 27535376

Abstract Author(s):

Dong Wang, Shi-Ping Li, Jin-Sheng Fu, Sheng Zhang, Lin Bai, Li Guo

Article Affiliation:

Dong Wang

Abstract:

The mouse autoimmune encephalomyelitis (EAE), an experimental model of multiple sclerosis (MS), is primarily characterized as dysfunction of the blood-brain barrier (BBB). Resveratrol exhibits anti-inflammatory, anti-oxidative and neuroprotective activities. We investigated the beneficial effects of resveratrol in protecting the integrity of the BBB in EAE mice, and observed improved clinical outcome in the EAE mice after resveratrol treatment. Evans blue (EB) extravasation was used to detect the disruption of BBB. Western blot were used to detected the tight junction proteins and adhesion molecules, zonula occluden-1, occludin, ICAM-1 and VCAM-1. Inflammatory factors iNOS, IL-1b and arginase1 were evaluated by qPCR, and IL-10 by ELISA. NADPH oxidase levels were evaluated by qPCR, and its activity was analyzed by lucigenin-derived chemiluminiscence. Resveratrol at doses of 25 and 50 mg/kg produced a dose-dependent decrease in EAE paralysis and EB leakage, ameliorated EAE-induced loss of tight junction proteins ZO-1, occludin and claudin-5, as well as repressed the EAE-induced increase in adhesion proteins ICAM-1 and VCAM-1. In addition, resveratrol suppressed the EAE-induced overexpression of pro-inflammatory transcripts iNOS and IL-1β, and upregulated the expression of anti-inflammatory transcripts arginase 1 and IL-10 cytokine in the brain. Furthermore, resveratrol downregulated the overexpressed NOX-2 and NOX-4 in the brain and suppressed NADPH activity. Resveratrol ameliorates the clinical severity of MS through maintainingthe BBB integrity in EAE mice.

Print Options


Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2019 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.