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Abstract Title:

[Radiation-induced G2 phase arrest may contribute to the radioresistance of breast cancer stem cells].

Abstract Source:

Nan Fang Yi Ke Da Xue Xue Bao. 2011 Jan;31(1):53-6. PMID: 21269956

Abstract Author(s):

Yun-hong Tian, Guo-zhu Xie, Chen Ren, Quan-quan Sun, Ai-min Sun, Ying Liu, Ya-wei Yuan

Article Affiliation:

Department of Radiation Oncology, Southern Medical University, Guangzhou 510515, China. tianyunhong1985@163.com

Abstract:

OBJECTIVE: To investigate radiation-induced cell cycle changes of human breast cancer stem cells enriched by suspension culture.

METHODS: The tumorigenicity of human breast cancer stem cell line MCF-7 cultured in serum-free media was confirmed in NOD/SCID mice, and the radiosensitivity of the cells was tested by clone formation assay following radiation exposure. Flow cytometry was performed to evaluate radiation-induced cell cycle changes, and the protein expression of pCDC25C (ser216) was measured by Western blotting.

RESULTS: After the exposure to 2 Gy radiation, the survived fraction of the cells in suspension culture and those in adherent culture was 0.856∓ 0.061 and 0.783 ∓ 0.097, respectively, and the cells in suspension culture showed an obviously greater capacity of tumorigenicity in NOD/SCID mice. The radiation exposure resulted in an obvious increase in the proportion of G2 phase cells from (22.03 ∓ 2.12)% to (45.83 ∓ 2.25)% and significantly increased the expression of pCDC25C (ser216).

CONCLUSION: Radiation- induced G2 phase arrest may contribute to the resistance of the breast cancer stem cells to radiotherapy.

Study Type : In Vitro Study
Additional Links
Anti Therapeutic Actions : Radiotherapy : CK(415) : AC(73)

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