The protective effects of sesamol in a neonatal rat model of necrotizing enterocolitis. - GreenMedInfo Summary
The protective effects of sesamol in a neonatal rat model of necrotizing enterocolitis.
J Matern Fetal Neonatal Med. 2018 Jul 29:1-11. Epub 2018 Jul 29. PMID: 30058400
Emine Burcu Çığşar
BACKGROUND AND AIM: Necrotizing enterocolitis (NEC) is a severe gastrointestinal inflammatory disease associated with high rates of morbidity and mortality. Its pathophysiology includes hypoxic-ischemic injury that may be related to oxygen-derived free radical formation. Sesamol is considered to be an antioxidant and free radical scavenger with anti-inflammatory effects. The aim of this study is to investigate the effects of sesamol in a neonatal rat model of NEC.
MATERIALS AND METHODS: The study included 1-d-old Wistar albino rat pups (n = 34) that were randomly divided into four groups: Group 1 (NEC), group 2 (NEC + intraperitoneal sesamol), group 3 (NEC + oral sesamol), and a control group. NEC was induced by exposure to hypoxia/reoxygenation, following cold stress and hyperosmolar enteral formula feeding. Sesamol 100 mgkgdosewas administered intraperitoneally to group 2 and orally to group 3 for 3 d. On d 4 all rats were sacrificed. Histological injuries, the Bcl-2, caspase-3, malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione-peroxidase (GSH-Px) activities were measured in intestinal samples.
RESULTS: The grade of intestinal damage, and Bcl-2 and caspase-3 levels in group 1 were significantly higher than in groups 2 and 3 and the control group, and intestinal damage was significantly more severe in group 1 than in groups 2 and 3. The MDA activity was significantly lower in groups 2 and 3 than in group 1 (112, 89, and 144 nmol mL, respectively). Groups 2 and 3 had significantly higher SOD and GSH-Px activities than group 1 (SOD: 1.75, 1.74 and 0.89 U mg; GSH- Px: 114, 121 and 110 nmol of NADPHminmg, respectively).
CONCLUSION: The present findings highlights that sesamol has beneficial effects on intestinal injury in a rat model of NEC through its antioxidant and anti inflammatory properties.