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Article Publish Status: FREE
Abstract Title:

The Metabolism of Polysaccharide from Atractylodes macrocephala Koidz and Its Effect on Intestinal Microflora.

Abstract Source:

Evid Based Complement Alternat Med. 2014 ;2014:926381. Epub 2014 Nov 19. PMID: 25505927

Abstract Author(s):

Ruijun Wang, Guisheng Zhou, Mengyue Wang, Ying Peng, Xiaobo Li

Article Affiliation:

Ruijun Wang

Abstract:

An active polysaccharide from the rhizome of Atractylodes macrocephala Koidz (PAM) was identified to improve and adjust disordered intestinal flora. High-performance gel permeation chromatography (HPGPC) and gas chromatography-mass spectrometry (GC-MS) were employed to identify the components of PAM as rhamnose, glucose, mannose, xylose, and galactose at a ratio of 0.03 : 0.25 : 0.15 : 0.41 : 0.15. PAM metabolized in gastrointestinal tract when incubated with artificial gastric and intestinal juices. Anaerobic incubation of PAM on intestinal flora confirmed that PAM promoted the ability of intestinal bacteria to digest reducing sugar. Based on theShannon index and similarity coefficient index of enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) fingerprinting of the total intestinal bacteria DNA, we concluded that PAM can significantly improve disordered intestinal flora and may be used as an oral adjuvant to regulate intestinalflora.

Study Type : Animal Study

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Sayer Ji
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