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Abstract Title:

The effect of phloretin on humanγδ T cells killing colon cancer SW-1116 cells.

Abstract Source:

Int Immunopharmacol. 2013 Jan ;15(1):6-14. Epub 2012 Nov 19. PMID: 23174508

Abstract Author(s):

Sheng-Ping Zhu, Gang Liu, Xiao-Ting Wu, Fu-Xing Chen, Jun-Quan Liu, Zhong-Hai Zhou, Jian-Fu Zhang, Su-Juan Fei

Article Affiliation:

Sheng-Ping Zhu

Abstract:

OBJECTIVE: To explore the effect and mechanism of Phloretin on humanγδ T cells killing colon cancer SW-1116 cells.

METHODS: γδ T cells were amplified in vitro from human peripheral blood mononuclear cells through isopentenyl pyrophosphate method (IPP). After cocultured different concentrations of Phloretin with γδ T cells or SW-1116 cells for 48h respectively, MTT assay was used to test the growth curve of these twocells; Flow cytometry to test the expression of Granzyme B (GraB), perforin (PFP), CD107a and IFN-γ of γδ T cells; Lactate dehydrogenase (LDH) release assay to test the cytotoxic activity of the γδ T cells on SW-1116 cells; and Western blot to test the Wnt3a expression of the γδ T cells.

RESULTS: After cultured with IPP for ten days, the percentage ofγδ T cells increased from 3.31±3.00% to 78.40±10.30%. Compared to the control group, when the concentration of Phloretin increased from 2.35μg/ml to 18.75μg/ml, it could significantly proliferate the γδ T cell growth (P<0.05) and inhibit the growth of SW-1116 cells in dose-response, and the expression of GraB, PFP, CD107a and Wnt3a significantly increased (P<0.05). Significant positive relationships were observed among CD107a and PFP, GraB, cytotoxicity (P<0.05). The percentage of IFN-γ producing γδ T cells treated with Phloretin was significantly higher than control group.

CONCLUSION: Phloretin can enhance the killing effect ofγδ T cells on SW-1116 cells; the mechanism may be that Phloretin could proliferate the γδ T cell growth, increase the expression of PFP and GraB, activate the Wnt signaling pathway, and produce higher level of IFN-γ. Indeed CD107a expression probably correlates quite well with antitumor activity.

Study Type : In Vitro Study
Additional Links
Pharmacological Actions : Immunomodulatory : CK(2249) : AC(733)

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