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Abstract Title:

Paeonia lactiflora Pall inhibits bladder cancer growth involving phosphorylation of Chk2 in vitro and in vivo.

Abstract Source:

J Ethnopharmacol. 2011 Apr 26 ;135(1):162-72. Epub 2011 Mar 17. PMID: 21396995

Abstract Author(s):

Ting-Tsz Ou, Cheng-Hsun Wu, Jeng-Dong Hsu, Charng-Cherng Chyau, Huei-Jane Lee, Chau-Jong Wang

Article Affiliation:

Ting-Tsz Ou

Abstract:

ETHNOPHARMACOLOGICAL RELEVANCE: Extracts of Paeonia lactiflora Pall (RPA), a traditional Chinese medicines has been shown to treat cancers.

AIM OF THE STUDY: The purpose of this study is to evaluate the anticancer effect of RPA in urinary bladder carcinoma in vitro and in vivo.

MATERIALS AND METHODS: The cell viability was analyzed with DAPI. Flow cytometry and Western blot were used to study the apoptosis and cell cycle related mechanism. A rat model of bladder cancer was induced by N-butyl-N-(4-hydroxybutyl) nitrosamine (OH-BBN). Tumors were analyzed with immunohistochemical analysis.

RESULTS: Our data suggested that RPA inhibits growth of bladder cancer via induction of apoptosis and cell cycle arrest. Treatment of TSGH-8301 cells with RPA resulted in G2-M phase arrest that was associated with a marked decline in protein levels of cdc2, cyclin B1, cell division cycle 25B (Cdc25B) and Cdc25C. We also reported that RPA-mediated growth inhibition of TSGH-8301 cells was correlated with activation of checkpoint kinase 2 (Chk2). Herein, we further evaluated urinary bladder cancer using a model of bladder cancer induced by OH-BBN. Analysis of tumors from RPA-treated rats showed significant decrease in the expression of Bcl2, cyclin D1, and PCNA, and increase in the expression of p-Chk2 (Thr-68), Bax, and Cip1/p21.

CONCLUSION: Our data provide the experimental evidence that RPA could modulate apoptosis in models of bladder cancer.

Study Type : Animal Study, In Vitro Study

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Sayer Ji
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