N-acetylcysteine protected against titanium dioxide's damaging effects. - GreenMedInfo Summary
Histopathological Effects of Titanium Dioxide Nanoparticles and The Possible Protective Role of N-Acetylcysteine on The Testes of Male Albino Rats.
Int J Fertil Steril. 2018 Jun ;12(3):249-256. Epub 2018 Jun 20. PMID: 29935072
Amir M Bassam Elnagar
BACKGROUND: Titanium dioxide (TiO) is a white pigment which is used in paints, plastics, etc. It is reported that TiOinduces oxidative stress and DNA damage. N-acetylcysteine (NAC) has been used to fight oxidative stress-induced damage in different tissues. The objective of this study was to evaluate the toxic effects of orally administered TiOnanoparticles and the possible protective effect of NAC on the testes of adult male albino rats.
MATERIALS AND METHODS: In this experimental study, 50 adult male albino rats were classified into five groups. Group I was the negative control, group II was treated with gum acacia solution , group III was treated with NAC, group IV was treated with TiOnanoparticles, and group V was treated with 100 mg/kg of NAC and 1200 mg/kg TiOnanoparticles. Total testosterone, glutathione (GSH), and serum malondialdehyde (MDA) levels were estimated. The testes were subjected to histopathological, electron microscopic examinations, and immunohistochemical detection for tumor necrosis factor (TNF)-α. Cells from the left testis were examined to detect the degree of DNA impairment by using the comet assay.
RESULTS: TiOnanoparticles induced histopathological and ultrastructure changes in the testes as well as positive TNF-α immunoreaction in the testicular tissue. Moreover, there was an increase in serum MDA while a decrease in testosterone and GSH levels in TiOnanoparticles-treated group. TiOresulted in DNA damage. Administration of NAC to TiO- treated rats led to improvement of the previous parameters with modest protective effects against DNA damage.
CONCLUSION: TiO-induced damage to the testes was mediated by oxidative stress. Notably, administration of NAC protected against TiO's damaging effects.