Down-Regulation of Hepatic de novo Lipogenesis and Adipogenesis in Adipocytes by Pinus densiflora Bark Extract.
J Microbiol Biotechnol. 2017 Sep 5. Epub 2017 Sep 5. PMID: 28870006
Korean red pine (Pinus densiflora) bark extract, PineXol (PX), was investigated on its potential anti-oxidant and anti-inflammation effects in vitro. It was hypothesized that PX treatment (25-150µg/mL) would reduce the lipid synthesis in HepG2 hepatocytes as well as lipid accumulation in 3T3-L1 adipocytes. Hepatocytes' intracellular triglycerides and cholesterol were decreased in the PX 150 µg/mL treatment group compared to the control (p<0.05). Consequently, de novo lipogenic proteins; acetyl-CoA carboxylase 1, stearoyl-CoA desaturase 1, elongase of very long chain fatty acids 6, glycerol-3-phosphate acyltransferase 1, and sterol regulatory element-binding protein 1, were significantly decreased in hepatocytes by PX 150µg/mL treatment compared to the control (p<0.05). In the differentiated 3T3-L1 adipocytes, the lipid accumulation was significantly attenuated by all PX treatments (p<0.01). Regulators of adipogenesis including CCAAT-enhancer-binding proteins alpha, peroxisome proliferator-activated receptor gamma and perilipin were decreased in PX 100µg/mL treatment compared to the control (p<0.05). In conclusion, PX might have anti-obese effects by blocking hepatic lipogenesis and by inhibiting adipogenesis in adipocytes.