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Abstract Title:

Antiproliferative effect of isolated isoquinoline alkaloid from Mucuna pruriens seeds in hepatic carcinoma cells.

Abstract Source:

Nat Prod Res. 2016 ;30(4):460-3. Epub 2015 Mar 16. PMID: 25774560

Abstract Author(s):

Pranesh Kumar, Atul Rawat, Amit K Keshari, Ashok K Singh, Siddhartha Maity, Arnab De, Amalesh Samanta, Sudipta Saha

Article Affiliation:

Pranesh Kumar

Abstract:

The present study was undertaken to investigate the antiproliferative action of isolated M1 (6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid) from Mucuna pruriens seeds using human hepatic carcinoma cell line (Huh-7 cells). Initially, docking studies was performed to find out the binding affinities of M1 to caspase-3 and 8 enzymes. Later, cytotoxic action of M1 was measured by cell growth inhibition (MTT), followed by caspase-3 and 8 enzymes assay colorimetrically. Our results collectively suggested that M1 had strong binding affinity to caspase-8 in molecular modelling. M1 possessed antiproliferative activity on Huh-7 cells (EC50 = 13.97μM) and also inhibited the action of caspase-8 enzyme, signified process of apoptosis. M1 was active against Huh-7 cells that may be useful for future hepatic cancer treatment.

Study Type : In Vitro Study

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Sayer Ji
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