[Effects of Honokiol on proliferation, migration and invasion in nasopharyngeal carcinoma cells].
Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2017 Jan 20 ;31(2):88-90. PMID: 29871193
To investigate the effects of Honokiol on proliferation, migration and invasion in nasopharyngeal carcinoma cell line CNE-1 and to explore the possible mechanism.Cell viability was assessed using MTT in CNE-1 cells treated with different concentrations of Honokio (0, 20, 40, 80 and 160μmol/L). Cell migration and invasion were analyzed by wound healing and Transwell assay. The protein expression of PTEN, p-Akt and p-mTOR were determined by Western Blot.MTT assay showed that Honokiol decreased CNE-1 cell viability in a concentration dependent manner. The maximal inhibitory effects reached at 80μmol/L. The ability of CNE-1 cell migration and invasion were significantly inhibited after Honokiol treatment. Moreover, western blotting revealed that Honokiol treatment resulted in an increase of PTEN protein expression and a decrease of p-Akt and p-mTOR expression.Honokiol inhibits nasopharyngeal carcinoma cell line CNE-1 proliferation, migration and invasion via inactivation of PTEN/PI3k/Akt signaling pathway.