Gastrodin exerts robust neuroprotection in the post-ischemic brain. - GreenMedInfo Summary
Gastrodin exerts robust neuroprotection in the postischemic brain via its protective effect against Zn-toxicity and its anti-oxidative effects in astrocytes.
Anim Cells Syst (Seoul). 2018 ;22(6):429-437. Epub 2018 Nov 30. PMID: 30533266
Gastrodin (GAS) is a predominant bioactive constituent of the Chinese herbal medicine Tianma (Blume). Many authors have reported GAS has the beneficial effect on diverse diseases of the CNS, including epilepsy, Alzheimer's disease, Parkinson's disease, and cerebral ischemia. Here, we report GAS exhibited a robust neuroprotective effect in an Sprague-Dawley rat model of stroke (transient middle cerebral artery occlusion, tMCAO), and show that the underlying molecular mechanism involves its protective effect against Zn-toxicity and its anti-oxidative effects in astrocytes. Intraperitoneal administration of GAS (40 mg/kg) after MCAO reduced mean infarct volume to 30.1 ± 5.9% of that of MCAO controls and this neuroprotective effect was accompanied by neurological function recoveries which was measured using modified neurological severity score (mNSS). Interestingly, GAS induced up-regulation and nucleartranslocation of Nrf2, and subsequently increased the expressions of anti-oxidative genes, such as, HO-1 and GCLM, in astrocytes. Furthermore, GAS co- or pre-treatment markedly suppressed Zn-induced cell death caused by excessive ROS production and PARP-1 induction. We found that GAS suppressed p67 expression and PAR formation in astrocytes, which might underlie the anti- Zn-toxicity and anti-oxidative effects of GAS in astrocytes. These findings indicate GAS protects astrocytes from Zn-induced toxicity and oxidative stress and these effects contribute to its neuroprotective effects in the postischemic brain.