Hepatoprotective Effect ofLeaf Extract and Its Various Fractions against Carbon Tetra Chloride Induced Hepatotoxicity in Rats.
Evid Based Complement Alternat Med. 2018 ;2018:3782768. Epub 2018 Dec 16. PMID: 30643530
Abdelaaty A Shahat
is traditionally used as an antipyretic, digestive, and diuretic agent. Its flowers possess free radical-scavenging, antioxidative, and hepatoprotective effects. We investigated the hepatoprotective potential ofleaf extract and its various fractions against hepatotoxicity in rats. Liver injury was stimulated by the oral administration of carbon tetrachloride (CCl; 2.5 mL/kg b.wt.). Male albino rats (n = 55) were distributed arbitrarily into 11 groups: Group I, normal control group; Group II, CCl(positive control group); Group III, CCl+ silymarin; Groups IV and V, CCl+ two doses of 250 and 500 mg/kg of the 80% methanolic extract ofleaves, respectively; Groups VI and VII, CCl+ 250 mg/kg and 500 mg/kg of the ethyl acetate fraction, respectively; Groups VIII and IX, CCl+ 250 and 500 mg/kg of the butanol fraction, respectively; and Groups X and XI, CCl+ 250 and 500 mg/kg of the aqueous fraction ofleaves, respectively. CCl-treated rats that were given 250 or 500 mg/kg of the methanol extract ofleaves, or its ethyl acetate, butanol, or aqueous fractions, had significantly lower levels of biochemical parameters such as alanine aminotransferase, aspartate transaminase, alkaline phosphate, total protein, gamma-glutamyl transferase, and bilirubin levels than those of the CClpositive group. However, the extract and fractions did not significantly affect lipid profiles. Thus, we conclude thatleaf extract and its fractions have a hepatoprotective effect against CCl-induced hepatotoxicity in rats.