Enhanced Rg3 negatively regulates Th1 cell responses. - GreenMedInfo Summary
Enhanced Rg3 negatively regulates Th1 cell responses.
J Ginseng Res. 2019 Jan ;43(1):49-57. Epub 2017 Aug 16. PMID: 30662293
Background: Korean Red Ginseng (KRG;Meyer) is a widely used medicinal herb known to exert various immune modulatory functions. KRG and one of its purified components, ginsenoside Rg3, are known to possess anti-inflammatory activities. How they impact helper T cell-mediated responses is not fully explored. In this study, we attempted to evaluate the effect of KRG extract (KRGE) and ginsenoside Rg3 on Th1 cell responses.
Methods: Using well-characterized T celldifferentiation systems, we examined the effects of KRGE or enhanced Rg3 on the Th1-inducing cytokine production from dendritic cells (DC) and the naïve CD4T cells differentiation to Th1 cells. Furthermore, we examined the change of Th1 cell population in the intestine after treatment of enhanced Rg3. The influence of KRGE or enhanced Rg3 on Th1 cell differentiation was evaluated by fluorescence-activated cell sorting, enzyme-linked immunosorbent assay, and quantitative real-time polymerase chain reaction.
Results: KRGE significantly inhibited the production level of IL-12 from DCs and subsequent Th1 cell differentiation. Similarly, enhanced Rg3 significantly suppressed the expression of interferon gamma (IFNγ) and T-bet in T cells under Th1-skewing condition. Consistent with these effects, oral administration of enhanced Rg3 suppressed the frequency of Th1 cells in the Peyer's patch and lamina propria cells.
Conclusion: Enhanced Rg3 negatively regulates the differentiation of Th1 celland Th1 cell responses in the gut, providing fundamental basis for the use of this agent to treat Th1-related diseases.