Development ofBiofilms Is Diminished byvia Obstruction of Cell Adhesion and Cell Lysis.
J Microbiol Biotechnol. 2018 Mar 28 ;28(3):482-490. PMID: 29316739
infections are often problematic to treat owing to antifungal resistance, as such infections are mostly associated with biofilms. The ability ofto switch from a budding yeast to filamentous hyphae and to adhere to host cells or various surfaces supports biofilm formation. Previously, the ethanol extract fromwas reported to inhibit cell wall synthesis and cause depolarization and permeabilization of the cell membrane in. In this study, theextract was found to significantly reduce the initial stage ofbiofilms from 12 clinical isolates by 38.4%. Thus, to assess the action mechanism, the effect of theextract on the adhesion ofcells to polystyrene and germ tube formation was investigated using a microscopic analysis. The density of the adherent cells was diminished following incubation with theextract in an acidic medium. Additionally, the-treatedcells were mostly composed of less virulent pseudohyphae, and ruptured debris was found in the serum-containing medium. A quantitative real-time PCR analysis indicated thatdownregulated the expression ofhypha-specific genes:by 65% (= 0.004),by 34.9% (= 0.001),by 29.2% (= 0.002), andby 37.5% (= 0.001), matching the microscopic analysis of theaction on biofilm formation. Therefore, the current findings demonstrate that theethanol extract is effective in inhibitingbiofilms in vitro, suggesting its therapeutic potential for the treatment of biofilm-associated infections.