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Abstract Title:

[Apoptosis in nasopharyngeal carcinoma cell line NCE induced by curcumin and its molecular mechanism].

Abstract Source:

Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2006 Aug;41(8):612-6. PMID: 17039805

Abstract Author(s):

Fu-wen Yang, Jin-zhong Huang, Xiao-lan Lin, Ze-nian Zhen, Xian-ming Chen

Article Affiliation:

Department of Otorhinolaryngology, Zhujiang Hospital, Nanfang Medical University, Guangzhou 510280, China. puwenyang@163.com

Abstract:

OBJECTIVE: To investigate the mechanism underlying the curcumin-induced apoptosis of nasopharyngeal carcinoma (NPC) cell line NCE cells.

METHODS: The characteristics of apoptosis were identified by observation acridine orange and ethidium bromide stains, ultrastructure assay, DNA fragmentation assay and TdT-mediated dUTP nick end labeling method (TUNEL). Mitochondrial membrane potential (delta psi m), activity of caspase-3, cytosol cytochrome C and expression of gene Fas were determined by flow cytometry (FCM), Western Blot and reverse transcription-polymerase chain reaction (RT-PCR).

RESULTS: Several evidences of apoptosis were obtained from curcumin-treated NCE cells by acridine orange and ethidium bromide stains, ultrastructure identification, DNA fragmentation assay and TUNEL staining. And the mean TUNEL-positive rates increased significantly at the 3 different time points (12 h, 24 h and 48 h; 25.6%, 40.3% and 54.5%, respectively). In the curcumin-treated-groups, delta psi m altered significantly and the positive rates increased in a time-dependent manner. At the 3 different time points, the mean positive rates were 26.8%, 42.3% and 68.2%, respectively. When caspase-3 activity was detected, 80.5% cells presented proteases activities after 12 h incubation with curcumin. Western Blot analysis showed that cytoplasmic cytochrome C increased significantly after incubation with curcumin. Flow cytometry and RT-PCR analysis showed that curcumin could up-regulate the Fas expression in time-depended manner , the positive rates of Fas protein increased from 33.6% to 89.9%.

CONCLUSIONS: Curcumin induced apoptosis of NCE cells both through mitochondria-dependent pathway and death receptor pathway.

Study Type : In Vitro Study
Additional Links
Pharmacological Actions : Apoptotic : CK(5217) : AC(3846)

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Sayer Ji
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