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Abstract Title:

The role of p53 in silica-induced cellular and molecular responses associated with carcinogenesis.

Abstract Source:

J Toxicol Environ Health A. 2009;72(23):1509-19. PMID: 20077225

Abstract Author(s):

Maureen R Gwinn, Stephen S Leonard, Linda M Sargent, David T Lowry, Kimberly McKinstry, Terry Meighan, Steve H Reynolds, Michael Kashon, Vince Castranova, Val Vallyathan

Article Affiliation:

National Institute for Occupational Safety and Health, Health Effects Laboratory Division, Morgantown, West Virginia, USA.

Abstract:

Crystalline silica (silica), a suspected human carcinogen, produces an increase in reactive oxygen species (ROS) when fractured using mechanical tools used in several occupations. Although ROS has been linked to apoptosis, DNA damage, and carcinogenesis, the role of enhanced ROS production by silica in silica-induced carcinogenesis is not completely understood. The goal of this study was to compare freshly fractured and aged silica-induced molecular alterations in human immortalized/transformed bronchial epithelial cells (BEAS-IIB) and lung cancer cells with altered (H460) or deficient (H1299) p53 expression. Exposure to freshly fractured or aged silica produced divergent cellular responses in certain downstream cellular events, including ROS production, apoptosis, cell cycle and chromosomal changes, and gene expression. ROS production increased significantly following exposure to freshly fractured silica compared to aged silica in BEAS-IIB and H460 cells. Apoptosis showed a comparable enhanced level of induction with freshly fractured or aged silica in both cancer lines with p53 functional changes. p53 protein was present in the BEAS-IIB and was absent in cancer cell lines after silica exposure. Exposure to freshly fractured silica also resulted in a rise in aneuploidy in cancer cells with a significantly greater increase in p53-deficient cells. Cytogenetic analysis demonstrated increased metaphase spreads, chromosome breakage, rearrangements, and endoreduplication in both cancer cells. These results suggest that altered and deficient p53 affects the cellular response to freshly fractured silica exposure, and thereby enhances susceptibility and augments cell proliferation and lung cancer development.

Study Type : In Vitro Study
Additional Links
Problem Substances : Silicon Dioxide : CK(4) : AC(4)

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Sayer Ji
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