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Abstract Title:

Effects of Resveratrol, Berberine, and Their Combinations on Reactive Oxygen Species, Survival, and Apoptosis in Human Squamous Carcinoma (SCC-25) Cells.

Abstract Source:

Anticancer Agents Med Chem. 2019 Apr 5. Epub 2019 Apr 5. PMID: 30950357

Abstract Author(s):

Magdalena Skonieczna, Dorota Hudy, Aleksandra Poterala-Hejmo, Tomasz Hejmo, Rafal J Buldak, Arkadiusz Dziedzic

Article Affiliation:

Magdalena Skonieczna

Abstract:

BACKGROUND: Levels of cellular Reactive Oxygen Species (ROS) influence the oxidized/reduced states of cellular proteins, and create redox-signaling pathways that can activate transcription factors, kinases, and phosphatases. ROS levels can be increased radically by external factors, including ionizing and UV radiation or exposure to chemical compounds. These increased ROS levels can in turn lead to oxidative damage of DNA. Natural plant treatments against cancer can modulate that processes by inducing or decreasing ROS production.

METHODS: Here we report new observations that squamous carcinoma (SCC-25) cells, exposed to 24 hours of combined resveratrol and berberine treatment contained increased ROS levels. Using flow cytometry, for drug activity characteristic, an accumulation of ROS was observed. Combination of different dyes, CellROX Green (Life Technologies) and DCFH-DA (Sigma), allowed for flow cytometric estimation of levels of cellular ROS as well as cellular localization.

RESULTS: Live staining and microscopic observations confirmed the accumulation of ROS in SCC-25 cells following combination treatment at concentrations of 10µg/ml. Additionally, the cytotoxicity of the compounds were significantly improved after their, combined application. Additive effects were observed for doses lower than the calculated IC50 of berberine [IC50=23 µg/ml] and resveratrol [IC50=9 µg/ml]. Viability (MTS) assays and analysis of isobolograms revealed a significant impact on cell viability upon combination treatment.

CONCLUSION: These results suggest that administration of berberine, in the presence of resveratrol, could be decreased even to 50% (half the IC50 for berberine) for cancer treatment.

Study Type : In Vitro Study

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Sayer Ji
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